Review





Similar Products

sino biological 40150 mm02  (Sino Biological)
94
Sino Biological sino biological 40150 mm02
Sino Biological 40150 Mm02, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s1+subunit/pmc12948273-281-60-60?v=Sino+Biological
Average 94 stars, based on 1 article reviews
sino biological 40150 mm02 - by Bioz Stars, 2026-08
94/100 stars
  Buy from Supplier
wild type sars cov 2 spike protein subunit s1  (Sino Biological)
95
Sino Biological wild type sars cov 2 spike protein subunit s1
Symmetric aptamer trimerization enhances binding affinity. ( A ) Schematic illustration of aptamer assembly. The 3′ end of monomeric aptamer MSA52t was linked to the trident linker (trebler phosphoramidite) with a 15-thymidine spacer (T15) and assembled into dimeric (shDMSA52t) or trimeric (shTMSA52t) constructs. This design provides flexible orientation and optimal target accessibility for each aptamer unit. ( B ) Dot-blot binding assays showing the affinities of MSA52t, shDMSA52t, and shTMSA52t toward the monomeric wild-type <t>S-protein</t> (mS-protein). Apparent dissociation constants ( K d ) were determined by nonlinear curve fitting. Trimeric assembly resulted in a ∼344-fold affinity enhancement over the monomer.
Wild Type Sars Cov 2 Spike Protein Subunit S1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s1+subunit/pmc13010139-39-1-20?v=Sino+Biological
Average 95 stars, based on 1 article reviews
wild type sars cov 2 spike protein subunit s1 - by Bioz Stars, 2026-08
95/100 stars
  Buy from Supplier
wild type s ar s cov 2 spike protein subunit s1  (Sino Biological)
95
Sino Biological wild type s ar s cov 2 spike protein subunit s1
Symmetric aptamer trimerization enhances binding affinity. ( A ) Schematic illustration of aptamer assembly. The 3′ end of monomeric aptamer MSA52t was linked to the trident linker (trebler phosphoramidite) with a 15-thymidine spacer (T15) and assembled into dimeric (shDMSA52t) or trimeric (shTMSA52t) constructs. This design provides flexible orientation and optimal target accessibility for each aptamer unit. ( B ) Dot-blot binding assays showing the affinities of MSA52t, shDMSA52t, and shTMSA52t toward the monomeric wild-type <t>S-protein</t> (mS-protein). Apparent dissociation constants ( K d ) were determined by nonlinear curve fitting. Trimeric assembly resulted in a ∼344-fold affinity enhancement over the monomer.
Wild Type S Ar S Cov 2 Spike Protein Subunit S1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s1+subunit/pm41873762-43-1-22?v=Sino+Biological
Average 95 stars, based on 1 article reviews
wild type s ar s cov 2 spike protein subunit s1 - by Bioz Stars, 2026-08
95/100 stars
  Buy from Supplier
sars cov 2 ntd subunit  (Sino Biological)
95
Sino Biological sars cov 2 ntd subunit
Symmetric aptamer trimerization enhances binding affinity. ( A ) Schematic illustration of aptamer assembly. The 3′ end of monomeric aptamer MSA52t was linked to the trident linker (trebler phosphoramidite) with a 15-thymidine spacer (T15) and assembled into dimeric (shDMSA52t) or trimeric (shTMSA52t) constructs. This design provides flexible orientation and optimal target accessibility for each aptamer unit. ( B ) Dot-blot binding assays showing the affinities of MSA52t, shDMSA52t, and shTMSA52t toward the monomeric wild-type <t>S-protein</t> (mS-protein). Apparent dissociation constants ( K d ) were determined by nonlinear curve fitting. Trimeric assembly resulted in a ∼344-fold affinity enhancement over the monomer.
Sars Cov 2 Ntd Subunit, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s1+subunit/pm41818265-194-18-21?v=Sino+Biological
Average 95 stars, based on 1 article reviews
sars cov 2 ntd subunit - by Bioz Stars, 2026-08
95/100 stars
  Buy from Supplier
sars cov 2 spike subunit 1  (ProSci Incorporated)
93
ProSci Incorporated sars cov 2 spike subunit 1
Symmetric aptamer trimerization enhances binding affinity. ( A ) Schematic illustration of aptamer assembly. The 3′ end of monomeric aptamer MSA52t was linked to the trident linker (trebler phosphoramidite) with a 15-thymidine spacer (T15) and assembled into dimeric (shDMSA52t) or trimeric (shTMSA52t) constructs. This design provides flexible orientation and optimal target accessibility for each aptamer unit. ( B ) Dot-blot binding assays showing the affinities of MSA52t, shDMSA52t, and shTMSA52t toward the monomeric wild-type <t>S-protein</t> (mS-protein). Apparent dissociation constants ( K d ) were determined by nonlinear curve fitting. Trimeric assembly resulted in a ∼344-fold affinity enhancement over the monomer.
Sars Cov 2 Spike Subunit 1, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s1+subunit/pmc12962466-20-0-5?v=ProSci+Incorporated
Average 93 stars, based on 1 article reviews
sars cov 2 spike subunit 1 - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier
recombinant sars cov 2 spike s1 subunit  (Sino Biological)
95
Sino Biological recombinant sars cov 2 spike s1 subunit
Symmetric aptamer trimerization enhances binding affinity. ( A ) Schematic illustration of aptamer assembly. The 3′ end of monomeric aptamer MSA52t was linked to the trident linker (trebler phosphoramidite) with a 15-thymidine spacer (T15) and assembled into dimeric (shDMSA52t) or trimeric (shTMSA52t) constructs. This design provides flexible orientation and optimal target accessibility for each aptamer unit. ( B ) Dot-blot binding assays showing the affinities of MSA52t, shDMSA52t, and shTMSA52t toward the monomeric wild-type <t>S-protein</t> (mS-protein). Apparent dissociation constants ( K d ) were determined by nonlinear curve fitting. Trimeric assembly resulted in a ∼344-fold affinity enhancement over the monomer.
Recombinant Sars Cov 2 Spike S1 Subunit, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s1+subunit/pmc12847319-56-13-18?v=Sino+Biological
Average 95 stars, based on 1 article reviews
recombinant sars cov 2 spike s1 subunit - by Bioz Stars, 2026-08
95/100 stars
  Buy from Supplier
recombinant sars cov 2 s1 protein  (R&D Systems)
94
R&D Systems recombinant sars cov 2 s1 protein
Symmetric aptamer trimerization enhances binding affinity. ( A ) Schematic illustration of aptamer assembly. The 3′ end of monomeric aptamer MSA52t was linked to the trident linker (trebler phosphoramidite) with a 15-thymidine spacer (T15) and assembled into dimeric (shDMSA52t) or trimeric (shTMSA52t) constructs. This design provides flexible orientation and optimal target accessibility for each aptamer unit. ( B ) Dot-blot binding assays showing the affinities of MSA52t, shDMSA52t, and shTMSA52t toward the monomeric wild-type <t>S-protein</t> (mS-protein). Apparent dissociation constants ( K d ) were determined by nonlinear curve fitting. Trimeric assembly resulted in a ∼344-fold affinity enhancement over the monomer.
Recombinant Sars Cov 2 S1 Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s1+subunit/pm41484280-85-21-25?v=R%26D+Systems
Average 94 stars, based on 1 article reviews
recombinant sars cov 2 s1 protein - by Bioz Stars, 2026-08
94/100 stars
  Buy from Supplier
mers-cov spike/s1 protein  (Sino Biological)
94
Sino Biological mers-cov spike/s1 protein
Symmetric aptamer trimerization enhances binding affinity. ( A ) Schematic illustration of aptamer assembly. The 3′ end of monomeric aptamer MSA52t was linked to the trident linker (trebler phosphoramidite) with a 15-thymidine spacer (T15) and assembled into dimeric (shDMSA52t) or trimeric (shTMSA52t) constructs. This design provides flexible orientation and optimal target accessibility for each aptamer unit. ( B ) Dot-blot binding assays showing the affinities of MSA52t, shDMSA52t, and shTMSA52t toward the monomeric wild-type <t>S-protein</t> (mS-protein). Apparent dissociation constants ( K d ) were determined by nonlinear curve fitting. Trimeric assembly resulted in a ∼344-fold affinity enhancement over the monomer.
Mers Cov Spike/S1 Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s1+subunit/custom%4040069-v08h%4010%2E1016%2Fj%2Emicroc%2E2026%2E117144?v=Sino+Biological
Average 94 stars, based on 1 article reviews
mers-cov spike/s1 protein - by Bioz Stars, 2026-08
94/100 stars
  Buy from Supplier
sars cov 2 elisa hcov nl63 spike s1 subunit  (Sino Biological)
93
Sino Biological sars cov 2 elisa hcov nl63 spike s1 subunit
Symmetric aptamer trimerization enhances binding affinity. ( A ) Schematic illustration of aptamer assembly. The 3′ end of monomeric aptamer MSA52t was linked to the trident linker (trebler phosphoramidite) with a 15-thymidine spacer (T15) and assembled into dimeric (shDMSA52t) or trimeric (shTMSA52t) constructs. This design provides flexible orientation and optimal target accessibility for each aptamer unit. ( B ) Dot-blot binding assays showing the affinities of MSA52t, shDMSA52t, and shTMSA52t toward the monomeric wild-type <t>S-protein</t> (mS-protein). Apparent dissociation constants ( K d ) were determined by nonlinear curve fitting. Trimeric assembly resulted in a ∼344-fold affinity enhancement over the monomer.
Sars Cov 2 Elisa Hcov Nl63 Spike S1 Subunit, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s1+subunit/pmc12625463-76-14-5?v=Sino+Biological
Average 93 stars, based on 1 article reviews
sars cov 2 elisa hcov nl63 spike s1 subunit - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier

Image Search Results


Symmetric aptamer trimerization enhances binding affinity. ( A ) Schematic illustration of aptamer assembly. The 3′ end of monomeric aptamer MSA52t was linked to the trident linker (trebler phosphoramidite) with a 15-thymidine spacer (T15) and assembled into dimeric (shDMSA52t) or trimeric (shTMSA52t) constructs. This design provides flexible orientation and optimal target accessibility for each aptamer unit. ( B ) Dot-blot binding assays showing the affinities of MSA52t, shDMSA52t, and shTMSA52t toward the monomeric wild-type S-protein (mS-protein). Apparent dissociation constants ( K d ) were determined by nonlinear curve fitting. Trimeric assembly resulted in a ∼344-fold affinity enhancement over the monomer.

Journal: Nucleic Acids Research

Article Title: A general strategy to enhance aptamer affinity by suppressing dissociation through symmetric assembly

doi: 10.1093/nar/gkag268

Figure Lengend Snippet: Symmetric aptamer trimerization enhances binding affinity. ( A ) Schematic illustration of aptamer assembly. The 3′ end of monomeric aptamer MSA52t was linked to the trident linker (trebler phosphoramidite) with a 15-thymidine spacer (T15) and assembled into dimeric (shDMSA52t) or trimeric (shTMSA52t) constructs. This design provides flexible orientation and optimal target accessibility for each aptamer unit. ( B ) Dot-blot binding assays showing the affinities of MSA52t, shDMSA52t, and shTMSA52t toward the monomeric wild-type S-protein (mS-protein). Apparent dissociation constants ( K d ) were determined by nonlinear curve fitting. Trimeric assembly resulted in a ∼344-fold affinity enhancement over the monomer.

Article Snippet: The wild-type SARS-CoV-2 spike protein subunit S1 (mS-protein, Cat. No. 40591-V08B1) and Troponin I (Cat. No. 501-TNNI0010) were purchased from Sino Biological Inc. VEGF 165 protein (his-tagged, Cat. No. VE5-H5248) was obtained from Acro Biosystems.

Techniques: Binding Assay, Construct, Dot Blot

Trimeric assembly enhances affinity primarily by reducing dissociation rate. Binding kinetics of ( A ) MSA52t, ( B ) shDMSA52t, and ( C ) shTMSA52t interacting with the wild-type SARS-CoV-2 S1-protein (mS-protein) were measured by BLI. Concentration-dependent sensorgrams were globally fitted using 1:1 binding model to obtain the kinetic parameters k on , k off , and K d . The experimental curves are shown as solid lines and the fitted curves are shown as black dashed lines. Bar graphs comparing ( D ) k on , ( E ) k off , and ( F ) K d values for each aptamer. While only modest changes in k on were observed, trimerization led to a dramatic reduction in k off , resulting in a 348-fold improvement in K d for shTMSA52t compared to the monomer.

Journal: Nucleic Acids Research

Article Title: A general strategy to enhance aptamer affinity by suppressing dissociation through symmetric assembly

doi: 10.1093/nar/gkag268

Figure Lengend Snippet: Trimeric assembly enhances affinity primarily by reducing dissociation rate. Binding kinetics of ( A ) MSA52t, ( B ) shDMSA52t, and ( C ) shTMSA52t interacting with the wild-type SARS-CoV-2 S1-protein (mS-protein) were measured by BLI. Concentration-dependent sensorgrams were globally fitted using 1:1 binding model to obtain the kinetic parameters k on , k off , and K d . The experimental curves are shown as solid lines and the fitted curves are shown as black dashed lines. Bar graphs comparing ( D ) k on , ( E ) k off , and ( F ) K d values for each aptamer. While only modest changes in k on were observed, trimerization led to a dramatic reduction in k off , resulting in a 348-fold improvement in K d for shTMSA52t compared to the monomer.

Article Snippet: The wild-type SARS-CoV-2 spike protein subunit S1 (mS-protein, Cat. No. 40591-V08B1) and Troponin I (Cat. No. 501-TNNI0010) were purchased from Sino Biological Inc. VEGF 165 protein (his-tagged, Cat. No. VE5-H5248) was obtained from Acro Biosystems.

Techniques: Binding Assay, Concentration Assay